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anti mgmt  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti mgmt
    Anti Mgmt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mgmt+antibody/bio_rxiv__64898__2026__04__02__716158-321-17-18
    Average 86 stars, based on 1 article reviews
    anti mgmt - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Temozolomide‐Promoted MGMT Transcription Contributes to Chemoresistance by Activating the ERK Signalling Pathway in Malignant Melanoma
    Article Snippet: The obtained proteins (50 μg) were separated on SurePAGE Bis‐Tris gradient concentration (4%–20%) SDS–PAGE gels (Genscript Biotech Corporation, Nanjing, China) and transferred to PVDF membranes. .. Membranes were blocked with 5% nonfat milk for 2 h at room temperature and then incubated with a rabbit anti–MEK1 antibody (CST, #9122), rabbit anti–p‐MEK1 antibody (CST, #9154), mouse anti–ERK1/2 antibody (CST, #4695), rabbit anti–p‐ERK1/2 antibody (CST, #4370), rabbit anti–p‐Chk1 antibody (CST, #2348), rabbit anti–p‐Chk2 antibody (CST, #82,263), rabbit anti–p‐ATR antibody (CST, #30,632), rabbit anti–p‐ATM antibody (CST, #6966), rabbit anti–γ‐H2AX (abcam, ab2893) antibody, rabbit anti–MGMT antibody (CST, #2739) or mouse anti–β‐actin antibody (CST, #8457) for 12 h at 4°C. .. Horseradish peroxidase–conjugated anti–mouse IgG (CST, #7076) or horseradish peroxidase–conjugated anti–rabbit IgG (CST, #7074) was used as a secondary antibody (at room temperature for 2 h), and antigen–antibody complexes were detected utilising an enhanced chemiluminescence kit (ECL Plus, Amersham, Freiburg, Germany).



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